کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1213000 | 1494117 | 2013 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Development and validation of an LC–MS/MS method for the determination of tolvaptan in human plasma and its application to a pharmacokinetic study Development and validation of an LC–MS/MS method for the determination of tolvaptan in human plasma and its application to a pharmacokinetic study](/preview/png/1213000.png)
Tolvaptan is a selective vasopressin V2-receptor antagonist mainly used for the treatment of hyponatremia. This study described the development and validation of an LC–MS/MS method for the determination of tolvaptan in human plasma. Sample preparation involved protein precipitation with acetonitrile containing 2-demethyl tolvaptan (internal standard, IS). Chromatographic separation was performed on a Zorbax XDB C18 column with an isocratic mobile phase consisting of water (containing 0.1% formic acid) and methanol (25:75, v/v). Determination of the analytes was achieved by tandem-mass spectrometry with positive electrospray ionization. The multiple reaction monitoring (MRM) transitions were performed at m/z 449.2 → 252.1 for tolvaptan and m/z 435.2 → 238.1 for IS. The assay was linear over the concentration range of 0.457–1000 ng/mL, with a lower limit of quantification of 0.457 ng/mL. The intra- and inter-day precisions at three concentration levels (0.914, 111 and 800 ng/mL) were less than 15% and their accuracies were within the range of 97.7–107.8%. The mean recovery ranged from 99.2 to 104.6% and the matrix effect from 89.3 to 99.5%. Tolvaptan was stable under all tested conditions. This validated method was successfully applied to a pharmacokinetic study in healthy volunteers after oral administration of single-dose tolvaptan tablets.
► A novel LC–MS/MS method for determination of tolvaptan in human plasma was developed.
► Simple protein precipitation was used for the sample preparation.
► The LLOQ of the method was 0.457 ng/mL, which was the lowest reported so far.
► The chromatographic run time was only 3.5 min.
► The method has been applied to a pharmacokinetic study of tovaptan in volunteers.
Journal: Journal of Chromatography B - Volumes 913–914, 15 January 2013, Pages 84–89