کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1213070 1494124 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Liquid chromatography–tandem mass spectrometry method for simultaneous determination of seven commonly used anticancer drugs in human plasma
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Liquid chromatography–tandem mass spectrometry method for simultaneous determination of seven commonly used anticancer drugs in human plasma
چکیده انگلیسی

This paper describes the development and validation of a novel, general liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the simultaneous determination of cyclophosphamide, ifosfamide, irinotecan, etoposide, gemcitabine, carboplatin and pemetrexed concentrations in human plasma. Samples were prepared by two kinds of extraction method and analyzed using a gradient separation over an Atlantis T3-C18 column (2.1 mm × 100 mm, 3 μm, Waters). Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile–water (0.1% formic acid and 10 mM ammonium acetate) at a flow rate of 0.25 mL/min. Linear coefficients of correlation were >0.992 for all analytes. The intra- and inter-day relative standard deviation across three validation runs over the entire concentration range was less than 9.2%, while the accuracy was within ±10.5%. The mean recovery of all the analytes ranged from 50.0 to 81.0%. This method was successfully applied to clinical samples from cancer patients.


► We developed a novel, sensitive, reproducible, and accurate LC–MS/MS method.
► This method could simultaneous determination of seven anticancer drugs in human plasma.
► This new method was successfully applied to clinical samples from cancer patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 906, 1 October 2012, Pages 1–8
نویسندگان
, , , , , , , ,