کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1213405 1494128 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The bioanalysis of the major Echinacea purpurea constituents dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides in human plasma using LC–MS/MS
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
The bioanalysis of the major Echinacea purpurea constituents dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides in human plasma using LC–MS/MS
چکیده انگلیسی

Alkylamides are a group of active components of the widely used herb Echinacea purpurea (E. purpurea), which have immunostimulatory and anti-inflammatory effects. For the most abundant alkylamides, dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides (DTAI), an LC–MS/MS assay has been developed and validated for quantification in human plasma. This assay will be used to support a clinical interaction study with E. purpurea. A 300 μL plasma aliquot underwent liquid–liquid extraction with diethylether-n-hexane (50:50, v/v). After evaporization and reconstitution in 100 μL of acetonitrile–water (50:50, v/v) 20 μL of sample were injected into the HPLC system. Chromatographic separation was achieved with a Polaris 3 C18-A column (50 mm × 2 mm ID, particle size 3 μm), a flow rate of 0.3 mL/min and isocratic elution with acetonitrile–water (50:50, v/v) containing 0.1% formic acid during the first 5 min. Hereafter, gradient elution was applied for 0.5 min, followed by restoration of the initial isocratic conditions. The total run time was 7.5 min. The assay was validated over a concentration range from 0.01 to 50 ng/mL for DTAI, with a lower limit of quantification of 0.01 ng/mL. Validation results show that DTAI can be accurately and precisely quantified in human plasma. DTAI also demonstrated to be chemically stable under relevant conditions. Finally, the applicability of this assay has been successfully demonstrated by measuring the plasma concentration of DTAI in patients after ingestion of a commercial extract of E. purpurea.


► An LC–MS/MS assay for the most abundant alkylamides (DTAI) in E. purpurea has been validated for quantification in human plasma.
► Most sensitive FDA-validated bioanalytical assay for DTAI with a quantification limit of 0.01 ng/mL.
► Only 300 μL plasma underwent simple liquid–liquid extraction. Run time was 7.5 min.
► DTAI was successfully quantified in patients after ingestion of E. purpurea extract.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 902, 1 August 2012, Pages 151–156
نویسندگان
, , , , , ,