کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1213698 1494122 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Capillary electrophoresis to quantitate gossypol enantiomers in cotton flower petals and seed
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Capillary electrophoresis to quantitate gossypol enantiomers in cotton flower petals and seed
چکیده انگلیسی

Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues including seed and flower petals. The (−)-enantiomer is more toxic to non-ruminant animals. Efforts to breed cottonseed with a low percentage of (−)-gossypol requires determination of the (+)- to (−)-gossypol ratio in seed and flower petals. We report a method to quantitatively determine the total gossypol and percent of its enantiomers in cotton tissues using high performance capillary electrophoresis (HPCE). The method utilizes a borate buffer at pH 9.3 using a capillary with internal diameter of 50 μm, effective length of 24.5 cm, 15 kV and cassette temperature of 15 °C. This method provides high accuracy and reproducible results with a limit of detection of the individual enantiomers of less than 36 ng/mL providing base line separation in less than 6 min.


► Gossypol occurs as a mixture of enantiomers in cottonseed.
► (−)-Gossypol is more toxic to non-ruminant animals.
► Plant breeders are developing plants with a low percent of (−)gossypol.
► A capillary electrophoresis method was developed to quantitate the enantiomers.
► Breeders can use this method to select plants with the low (−)gossypol seed trait.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 908, 1 November 2012, Pages 94–97
نویسندگان
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