کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1214341 | 1494158 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Quantification of raltegravir (MK0518) in human plasma by high-performance liquid chromatography with photodiode array detection Quantification of raltegravir (MK0518) in human plasma by high-performance liquid chromatography with photodiode array detection](/preview/png/1214341.png)
A precise and accurate high-performance liquid chromatography (HPLC) method with photodiode array detection has been developed and validated for raltegravir, a human immunodeficiency virus integrase strand transfer inhibitor (HIV-1 INSTI). Plasma (300 μL) was extracted with dichloromethane/hexane 50:50 (v/v) after addition of the internal standard, 6,7-dimethyl-2,3-di(2-pyridyl) quinoxaline. The compounds were separated using a dC18 column and detected with ultraviolet detection at 320 nm. The limit of quantification was 10 ng/mL for raltegravir. The method was linear and validated over a concentration range of 0–10,000 ng/mL. The intra-day precision ranged from 3.1 to 12.3%, while the intra-day accuracy ranged from −15.0 to −0.5%, the inter-day precision and accuracy were less than 7%. The mean recovery was 76.8%. Application to clinical samples taken from patients treated with raltegravir indicated that the method is suitable for measuring plasma concentrations of raltegravir in pharmacokinetic studies of clinical trials.
Journal: Journal of Chromatography B - Volume 878, Issues 3–4, 1 February 2010, Pages 456–460