کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1214686 | 1494145 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Capillary electrophoresis with contactless conductivity detection for the determination of carnitine and acylcarnitines in clinical samples Capillary electrophoresis with contactless conductivity detection for the determination of carnitine and acylcarnitines in clinical samples](/preview/png/1214686.png)
A capillary electrophoresis method with contactless conductivity detection was developed for the quantification of carnitine and six acylcarnitines in plasma and urine samples. The running buffer employed consisted of 500 mmol/L acetic acid, 1.0 mmol/L hydroxypropyl-β-cyclodextrin and 0.05% Tween at a pH of 2.6. Under these conditions, the isomeric valproyl- and octanoyl-carnitines could be distinguished. The linearity was in the range from 5.0 to 200.0 μmol/L with correlation coefficients between 0.9992 and 0.9997. The limits of detection were between 1.0 and 3.2 μmol/L. Intra- and inter-day precisions as %RSD were better than 10%. The method allows for direct determination without derivatisation or extraction processes. The method was applied for the quantification of carnitine and acetylcarnitine in plasma pre- and post-exercise, and to measure valproylcarnitine in plasma and urine of patients undergoing valproate therapy.
Journal: Journal of Chromatography B - Volume 879, Issues 13–14, 15 April 2011, Pages 921–926