Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin

• A simple and efficient method for preparative separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng was established.
• NKA-II resin was chosen and its condition for purification was optimized.
• The purity of chlorogenic acid in the product was 22.17%, with a recovery yield of 82.41%.
• The equilibrium adsorption data of NKA-II resin were fitted to Langmuir isotherm.

A simple and efficient chromatographic method for separation of chlorogenic acid from Eupatorium adenophorum Spreng extract was developed. The adsorption properties of nine macroporous resins were evaluated. NKA-II resin showed much better adsorption/desorption properties. The adsorption of chlorogenic acid on NKA-II resin at 25 °C was well fitted to Langmuir isotherm model and pseudo-second-order kinetic model. The dynamic adsorption and desorption experiments were carried out on columns packed with NKA-II resin to optimize the separation process. The content of chlorogenic acid in the product increased to 22.17%, with a recovery yield of 82.41%.