کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1214885 | 1494171 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Utilizing a â100 °C microplate CCD Imager, yttrium silicate coated 384-microplates and ultra-performance liquid chromatography for improved profiling of radiolabeled drug metabolites in complex biological samples
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The recent commercial availability of small particle packed columns (<2 μm) and associated instrumentation capable of withstanding the high pressures of such columns, has lead to an increase in the application of so called ultra-performance liquid chromatography. The improved efficiency, resolution and peak capacity of these columns, when coupled to mass spectrometry, provides particular benefit for the identification of drug metabolites in complex biological samples. In this work, the ability of ViewLuxâ¢, a microplate imager, to act as a suitable radiodetection system for ultra-performance liquid chromatography methods is assessed. The system demonstrates robustness and sensitivity comparable to a microplate scintillation counter (TopCount®) more typically used for off-line metabolite radiodetection. The ViewLux is also used here to undertake successful metabolite profiling of actual samples, for two investigational drug candidates, using both 96- and 384-well yttrium silicate microplates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 868, Issues 1â2, 1 June 2008, Pages 49-57
Journal: Journal of Chromatography B - Volume 868, Issues 1â2, 1 June 2008, Pages 49-57
نویسندگان
G.J. Dear, N. Patel, Alan Weightman, Hans Pirard, Miika Talvitie,