کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1215377 966973 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Label-free and amplified quantitation of proteins in complex mixtures using diffractive optics technology
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Label-free and amplified quantitation of proteins in complex mixtures using diffractive optics technology
چکیده انگلیسی

Immunoaffinity approaches remain invaluable tools for characterization and quantitation of biopolymers. Their application in separation science is often limited due to the challenges of immunoassay development. Typical end-point immunoassays require time consuming and labor-intensive approaches for optimization. Real-time label-free analysis using diffractive optics technology (dot®) helps guide a very effective iterative process for rapid immunoassay development. Both label-free and amplified approaches can be used throughout feasibility testing and ultimately in the final assay, providing a robust platform for biopolymer analysis over a very broad dynamic range. We demonstrate the use of dot in rapidly developing assays for quantitating (1) human IgG in complex media, (2) a fusion protein in production media and (3) protein A contamination in purified immunoglobulin preparations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 878, Issue 2, 15 January 2010, Pages 264–270
نویسندگان
, , ,