کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1215474 1494181 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simple and rapid quantitative high-performance liquid chromatographic analysis of plasma amino acids
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Simple and rapid quantitative high-performance liquid chromatographic analysis of plasma amino acids
چکیده انگلیسی

A simple and rapid high performance liquid chromatographic method for the determination of plasma amino acids was developed. The method uses minimal sample volume and automated online precolumn derivitization of amino acids with o-phthalaldehyde and fluorescent detection. Amino acids are separated by a simplified gradient without column heating. The assay is linear from 5 to 1000 μmol/L for all amino acids. Recovery of amino acids was between 91 and 108%, intra-assay coefficient of variation (CV) was 1–7%, and inter-assay CV was 2–12%. The simple sample preparation and minimal sample volume make the method useful for the quantitation of amino acids in both patient and experimental animal samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 852, Issues 1–2, 1 June 2007, Pages 646–649
نویسندگان
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