کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1215478 | 1494181 | 2007 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Single quadrupole mass spectrometry for pre-clinical pharmacokinetic analysis: Quantitation of carvedilol in dog plasma Single quadrupole mass spectrometry for pre-clinical pharmacokinetic analysis: Quantitation of carvedilol in dog plasma](/preview/png/1215478.png)
The pharmaceutical industry standard for bioanalysis is LC/MS/MS. There are, however, many instances where a single quadrupole detector could successfully be used to provide adequate sensitivity and selectivity for quantitation of drug substances in biological matrices. This paper presents one example of how a single quadrupole detector can be employed in a sensitive and selective analytical method for quantitation of carvedilol. A Synergi Hydro-RP (50 mm × 2 mm i.d.; 4 μm) column was used with acetonitile:water:formic acid mobile phase (32:68:0.01, v/v) at a flow rate of 200 μL/min into a single quadrupole mass spectrometer with an electrospray interface in the positive SIM mode. Using a 300 μL plasma aliquot and a liquid–liquid extraction procedure the limit of quantitation for the assay was 1 ng/mL. The assay utility was demonstrated in the analysis of carvedilol pharmacokinetic profiles in beagle dogs following oral carvedilol administration.
Journal: Journal of Chromatography B - Volume 852, Issues 1–2, 1 June 2007, Pages 665–668