کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1215658 1494183 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of the DNA methylation level of the marbled crayfish: An increase in sample throughput by an optimised sample preparation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of the DNA methylation level of the marbled crayfish: An increase in sample throughput by an optimised sample preparation
چکیده انگلیسی

Using a previously described capillary electrophoretic method with laser-induced fluorescence detection the genomic methylation level can be determined exactly. We present a sample preparation that eliminates the surplus of fluorescence marker used for coupling resulting in an increase of sample throughput from 75 to 250 analyses per week. The sensitivity of the method was also increased, which allows the determination of methylation levels under 1%. With these changes in sample preparation a methylation level of 1.64 ± 0.03% in hepatopancreas DNA of the recently discovered marbled crayfish could be determined.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 850, Issues 1–2, 1 May 2007, Pages 548–552
نویسندگان
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