کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1215695 | 966981 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Neural stem cell separation from the embryonic avian olfactory epithelium by sedimentation field-flow fractionation Neural stem cell separation from the embryonic avian olfactory epithelium by sedimentation field-flow fractionation](/preview/png/1215695.png)
The aim of the present study was to isolate neural stem cells from a complex tissue: the avian olfactory epithelium; by using sedimentation field flow fractionation (SdFFF). By using “Hyperlayer” elution mode, fraction collection and cell characterization methods, results shows that SdFFF could be a useful cell sorter to isolate an enriched, viable and sterile immature neural cell fraction from which the reconstitution of a complete epithelium was possible. In culture, SdFFF eluted cells first led to a “pseudoplacodal” epithelioid cell type from which derived “floating cells”. These cells were then able to generate neurosphere-like structures which were composed of cell having many features of immature cells: undifferentiated, self-renewable and multipotentiality. Such a population might be used as a model to improve our understanding of the mechanisms of olfactory neoneurogenesis.
Journal: Journal of Chromatography B - Volume 843, Issue 2, 7 November 2006, Pages 175–182