کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1215773 966983 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel method to detect seven microcystins in hard clam and corbicula fluminea by liquid chromatography–tandem mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A novel method to detect seven microcystins in hard clam and corbicula fluminea by liquid chromatography–tandem mass spectrometry
چکیده انگلیسی

A simple and reliable method to detect seven microcystins in hard clam and corbicula fluminea, based on liquid chromatography with electrospray ionization and tandem mass spectrometry (LC–ESI-MS/MS), was developed and validated. The sample preparation procedure includes extraction of tissue by methanol, followed by cleanup on a reversed-phase solid phase extraction (SPE) cartridge. With the optimized method, recoveries were between 43.7% and 92.3% for hard clam, 54.3% and 93.8% for corbicula fluminea, the relative standard deviations (RSD) were less than or equal to 16.2% and 15.7% in hard clam and corbicula fluminea at spiking levels of 1 μg/kg, 2 μg/kg and 5 μg/kg for MC-RR, MC-YR, MC-LR, and MC-LY, and 2 μg/kg, 5 μg/kg and 10 μg/kg for MC-LA, MC-LW and MC-LF, respectively, the limits of quantitation (LOQ) of this method were ranged from 0.7 μg/kg to 2.0 μg/kg.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 877, Issue 29, 1 November 2009, Pages 3522–3528
نویسندگان
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