کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1215840 | 966984 | 2007 | 11 صفحه PDF | دانلود رایگان |

A fast, sensitive and specific LC–MS/MS bioanalytical method for the determination of unchanged clopidogrel in human plasma has been developed and validated over the range of 10–12,000 pg mL−1 (r2 0.9993) by the Contract Research group at HFL. Samples (0.3 mL) were buffered (pH 6.8), extracted using diethyl ether and 10 μL of the sample extract was injected onto the LC–MS/MS system. Analysis was performed using a C8 column (temperature controlled to 50 °C) by gradient elution at a flow rate of 0.9 mL min−1 over a 3 min run time. Retention times of 1.61 and 1.59 min were observed for clopidogrel and 2H3-clopidogrel (I.S.), respectively. Detection was achieved using a Sciex API 4000, triple quadrupole mass spectrometer, in positive TurboIonspray™ (electrospray) ionisation mode. Ion transitions were monitored using MRM (multiple reaction monitoring) for clopidogrel (m/z 322–212) and for 2H3-clopidogrel (m/z 327–217). This validated method was used to support a pharmacokinetic study in healthy volunteers.
Journal: Journal of Chromatography B - Volume 848, Issue 2, 1 April 2007, Pages 344–354