کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1216041 | 1494160 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A sensitive and high-throughput LC–MS/MS method for the quantification of pegylated-interferon-α2a in human serum using monolithic C18 solid phase extraction for enrichment A sensitive and high-throughput LC–MS/MS method for the quantification of pegylated-interferon-α2a in human serum using monolithic C18 solid phase extraction for enrichment](/preview/png/1216041.png)
The analysis of pegylated-interferon-α2a in patient serum samples is of high interest for clinic research trials, as this therapeutic protein has become an important antiviral treatment. In this study, an LC–MS/MS method for the absolute quantification of pegylated-interferon-α2a in human serum was developed. The assay achieved a lower limit of quantification of 3.6 ng/mL (60 pM) with the use of a monolithic C18 solid phase extraction to enrich the target protein. The linear range of the assay was defined up to 54 ng/mL to measure the typical clinical pegylated-interferon-α2a levels, and within this range, the precision and accuracy were found to be within ±20%. The method was applied to a clinical study and found suitable for high-throughput analysis of pegylated-interferon-α2a in human serum. In addition, further investigations suggested the enrichment step may have general application to the sensitive analysis of other low molecular weight proteins.
Journal: Journal of Chromatography B - Volume 877, Issues 18–19, 15 June 2009, Pages 1737–1742