کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1216057 | 966994 | 2006 | 5 صفحه PDF | دانلود رایگان |
Phytosterols (β-sitosterol, cholestanol and campesterol) and cholesterol precursors (desmosterol and lathosterol), have been suggested as important biochemical markers of intestinal cholesterol absorption and liver biosynthesis, respectively, and as useful clinical parameters in the study of hypercholesterolemia, β-sitosterolemia, atherosclerosis and cardiovascular disease, including pharmacological response to hypolipidemic agents. We developed an optimised analytical method for the simultaneous analysis of cholestanol, desmosterol, lathosterol, campesterol and β-sitosterol in plasma using capillary gas chromatography coupled to mass spectrometry (GC–MS) with multiple selected ion monitoring (SIM). This method is based on the alkaline hydrolysis of sterol esters, extraction of free sterols and derivatization. The recovery of all sterols was in the range 76–101%. Within-day relative standard deviations (R.S.Ds.) and the between-day R.S.Ds. of cholestanol, desmosterol, lathosterol, campesterol and β-sitosterol were less than 8%, and their plasma levels in 161 normal subjects were (mean ± S.D.) 4.73 ± 2.57, 2.37 ± 1.04, 6.23 ± 3.14, 3.67 ± 1.95 and 5.92 ± 3.62 μmol/l, respectively.
Journal: Journal of Chromatography B - Volume 842, Issue 1, 14 September 2006, Pages 43–47