کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1216061 | 966994 | 2006 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: On-line biological sample cleanup for electrospray mass spectrometry using sol–gel columns On-line biological sample cleanup for electrospray mass spectrometry using sol–gel columns](/preview/png/1216061.png)
Using a slight overpressure, a urine sample is loaded onto a monolithic photopolymerized sol–gel column that has been derivatized with hydrophobic carbon chains and then the complex urine matrix is washed with aqueous solution. A buffer containing organic solvent is used to elute the adsorbed peptides by an applied voltage and the sample is then introduced into a mass spectrometer by sheath flow electrospray. The importance of desalting this type of sample is demonstrated by an experiment that shows that the signal intensity of a test solution with neurotensin, sprayed directly into the mass spectrometer, decreased from 4.5 × 104 cps to no detectible signal when just 10% urine is added to the sample solution. We suggest that this procedure may find general application for desalting biological samples prior to mass spectrometric analysis.
Journal: Journal of Chromatography B - Volume 842, Issue 1, 14 September 2006, Pages 70–74