کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1216383 | 967009 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A LC–MS/MS method to quantify the novel cholesterol lowering drug ezetimibe in human serum, urine and feces in healthy subjects genotyped for SLCO1B1 A LC–MS/MS method to quantify the novel cholesterol lowering drug ezetimibe in human serum, urine and feces in healthy subjects genotyped for SLCO1B1](/preview/png/1216383.png)
Ezetimibe (Ezetrol®) is a novel cholesterol lowering drug which disposition is not fully understood in man. We developed a selective and high-sensitive assay to measure serum concentration–time profiles, renal and fecal elimination of ezetimibe in pharmacokinetic studies. Ezetimibe glucuronide, the major metabolite of ezetimibe was determined by enzymatic degradation to the parent compound. Ezetimibe was measured after extraction with methyl tert-butyl ether using 4-hydroxychalcone as internal standard and liquid chromatography coupled via an APCI interface with tandem mass spectrometry (LC–MS/MS) for detection. The chromatography (column XTerra® MS, C18, 2.1 mm × 100 mm, particle size 3.5 μm) was done isocratically with acetonitrile/water (60/40, v/v; flow rate 200 μl/min). The MS/MS analysis was performed in the negative ion mode (m/z transition: ezetimibe 408–271, internal standard 223–117). The validation ranges for ezetimibe and total ezetimibe were as follows: serum 0.0001–0.015 μg/ml and 0.001–0.2 μg/ml; urine and fecal homogenate 0.025–10 μg/ml and 0.1–20 μg/ml, respectively. The assay was successfully applied to measure ezetimibe disposition in two subjects genotyped for the hepatic uptake transporter SLCO1B1.
Journal: Journal of Chromatography B - Volume 830, Issue 1, 2 January 2006, Pages 143–150