کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1216551 1494188 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Liquid chromatography–tandem mass spectrometric assay for the analysis of uracil, 5,6-dihydrouracil and β-ureidopropionic acid in urine for the measurement of the activities of the pyrimidine catabolic enzymes
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Liquid chromatography–tandem mass spectrometric assay for the analysis of uracil, 5,6-dihydrouracil and β-ureidopropionic acid in urine for the measurement of the activities of the pyrimidine catabolic enzymes
چکیده انگلیسی

A liquid chromatography–tandem mass spectrometric assay for the determination of uracil, 5,6-dihydrouracil and β-ureidopropionic acid in urine was developed to measure the activities of enzymes involved in pyrimidine breakdown. The assay was required to investigate the relation between the uracil–dihydrouracil ratio and toxicities observed after treatment with fluoropyrimidines drugs. After addition of stable isotopically labelled internal standards, the analytes were isolated from a 100-μl urine sample using liquid–liquid extraction with ethyl acetate–2-propanol. Compounds were separated on an Atlantis dC18 column, using ammonium acetate–formic acid in water as the eluent. The eluate was totally led into an electrospray interface with positive ionisation and the analytes were quantified using triple quadrupole mass spectrometry. The assay was validated in the range 1.6–1600 μM, using both, artificial urine and pooled urine as matrices. Intra-day precisions were ≤8% and inter-day precisions were ≤10%. Accuracies between 91 and 108% were found. The analytes were chemically stable under all relevant conditions and the assay was successfully applied in two clinical studies of cancer patients treated with 5-fluorouracil or capecitabine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 839, Issues 1–2, 24 July 2006, Pages 45–53
نویسندگان
, , , , ,