Urinalysis of 4-hydroxynonenal, a marker of oxidative stress, using stir bar sorptive extraction–thermal desorption–gas chromatography/mass spectrometry

A simple and fast method for the measurement of 4-hydroxynonenal (4HNE), a highly toxic end-product of lipid peroxidation, in urine samples is described. The method combines stir bar sorptive extraction (SBSE) with two derivatization steps, followed by thermal desorption and GC/MS. 4HNE is derivatized in situ with O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine and the oxime is extracted from the aqueous phase with SBSE. The 4HNE-oxime is further acylated by headspace derivatization prior to thermal desorption. Derivatization reactions and extraction were optimized in terms of reagent quantities, temperature and time. The method is linear over a concentration range of 0.5–5 ng mL−1 with a correlation coefficient of 0.997. The limit of detection and limit of quantitation are 22 and 75 pg mL−1 urine, respectively. The high sensitivity of the method allows the measurement of physiological concentrations of 4HNE in urine samples.