کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217106 | 1494177 | 2007 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Structural effect of a recombinant monoclonal antibody on hinge region peptide bond hydrolysis Structural effect of a recombinant monoclonal antibody on hinge region peptide bond hydrolysis](/preview/png/1217106.png)
IgG hinge region peptide bonds are susceptible to degradation by hydrolysis. To study the effect of Fab and Fc on hinge region peptide bond hydrolysis, a recombinant humanized monoclonal IgG1 antibody, its F(ab′)2 fragment, and a model peptide with amino acid sequence corresponding to the hinge region were incubated at 40 °C in formulation buffer including complete protease inhibitor and EDTA for 0, 2, 4, 6 and 8 weeks. Two major cleavage sites were identified in the hinge region of the intact recombinant humanized monoclonal antibody and its F(ab′)2 fragment, but only one major cleavage site of the model peptide was identified. Hinge region peptide bond hydrolysis of the intact antibody and its F(ab′)2 fragment degraded at comparable rates, while the model peptide degraded much faster. It was concluded that Fab region of the IgG, but not Fc portion had significant effect on preventing peptide bond cleavage by direct hydrolysis. Hydrolysis of hinge region peptide bonds was accelerated under both acidic and basic conditions.
Journal: Journal of Chromatography B - Volume 858, Issues 1–2, 15 October 2007, Pages 254–262