کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1217139 | 967068 | 2011 | 6 صفحه PDF | دانلود رایگان |
A rapid and sensitive method based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) for the simultaneous determination of codeine, ephedrine, guaiphenesin and chlorpheniramine in beagle dog plasma has been developed and validated. Following liquid–liquid extraction, the analytes were separated on a reversed-phase C18 column (150 mm × 2.0 mm, 3 μm) using formic acid:10 mM ammonium acetate:methanol (0.2:62:38, v/v/v) as mobile phase at a flow rate of 0.2 mL/min and analyzed by a triple-quadrupole mass spectrometer in the selected reaction monitoring (SRM) mode. The method was linear for all analytes over the following concentration (ng/mL) ranges: codeine 0.08–16; ephedrine 0.8–160; guaiphenesin 80–16,000; chlorpheniramine 0.2–40. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. It is the first time that the validated HPLC–MS/MS method was successfully applied to a bioequivalence study in 6 healthy beagle dogs.
► This is the first study to describe a sensitive and precise LC–MS/MS assay for codeine, ephedrine, guaiphenesin and chlorpheniramine.
► We utilized this method to measure concentration of codeine, ephedrine, guaiphenesin and chlorpheniramine in beagle dog plasma.
► This method supports research of codeine, ephedrine, guaiphenesin and chlorpheniramine and its pharmacokinetics in beagle dog plasma.
Journal: Journal of Chromatography B - Volume 879, Issue 32, 15 December 2011, Pages 3937–3942