کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217176 1494185 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fast differentiation of meats from fifteen animal species by liquid chromatography with electrochemical detection using copper nanoparticle plated electrodes
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fast differentiation of meats from fifteen animal species by liquid chromatography with electrochemical detection using copper nanoparticle plated electrodes
چکیده انگلیسی

A simple, rapid and reliable method based on high-performance liquid chromatography with electrochemical detection was developed to routinely differentiate among meat products from fifteen food animal species. Samples from cattle, pigs, goats, deer, horses, chickens, ducks, ostriches, salmon, cod, shrimp, crabs, scallops, bullfrogs and alligators each exhibited unique electrochemical profiles. Species-specific markers exhibited reproducible peak retention times with coefficients of variation less then 6% across different runs, body regions and subjects. The method requires no derivatization or extraction steps and may be applicable to fresh or cooked meats. Incubation of fresh beef, pork or chicken at room temperature for 24 h or repeated freezing and thawing changed the intensity but not the pattern of species-specific peaks. In conclusion, this method appears suitable for rapid differentiation of meats from various food animal species and demonstrates the utility of electrochemical detection to supplement existing immunochemical and molecular biological methods. The possibility of using this method to detect adulteration and degradative changes of meat proteins is discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 846, Issues 1–2, 1 February 2007, Pages 230–239
نویسندگان
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