کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217358 1494190 2006 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Screening for basic drugs in equine urine using direct-injection differential-gradient LC–LC coupled to hybrid tandem MS/MS
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Screening for basic drugs in equine urine using direct-injection differential-gradient LC–LC coupled to hybrid tandem MS/MS
چکیده انگلیسی

A rapid, selective and robust direct-injection LC/hybrid tandem MS method has been developed for simultaneous screening of more than 250 basic drugs in the supernatant of enzyme hydrolysed equine urine. Analytes, trapped using a short HLB® extraction column, are refocused and separated on a Sunfire® C18 analytical column using a controlled differential gradient generated by proportional dilution of the first column's eluent with water. Independent data acquisition (IDA) was configured to trigger a sensitive enhanced product ion (EPI) scan when a multiple reaction monitoring (MRM) survey scan signal exceeded the defined criteria. The decision on whether or not to report a sample as a positive result was based upon both the presence of a MRM response within the correct retention time range and a qualitative match between the EPI spectrum obtained and the corresponding reference standard. Ninety seven percent of the drugs targeted by this method met our detection criteria when spiked into urine at 100 ng/ml; 199 were found at 10 ng/ml, 83 at 1 ng/ml and 4 at 0.1 ng/ml.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 836, Issues 1–2, 19 May 2006, Pages 1–14
نویسندگان
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