کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217446 | 1494193 | 2006 | 9 صفحه PDF | دانلود رایگان |
The metabolism of zebularine (NSC 309132), a novel agent that inhibits DNA methyltransferases, is still uncharacterized. To examine the in vivo metabolism of zebularine, an analytical method was developed and validated (based on FDA guidelines) to quantitate 2-[14C]-zebularine and its major metabolites in murine plasma. Zebularine and its metabolites uridine, uracil and dihydrouracil were baseline-separated based on hydrophilic interaction chromatography by using an amino column. The assay was accurate and precise in the concentration ranges of 5.0–100 μg/mL for zebularine, 2.5–50 μg/mL for uridine, 1.0–10 μg/mL for uracil and 0.5–5.0 μg/mL for dihydrouracil. This assay is being used to quantitate zebularine and its metabolites in ongoing pharmacokinetic studies of zebularine.
Journal: Journal of Chromatography B - Volume 831, Issues 1–2, 2 February 2006, Pages 147–155