کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217449 1494193 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of lapatinib (GW572016) in human plasma by liquid chromatography electrospray tandem mass spectrometry (LC–ESI-MS/MS)
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of lapatinib (GW572016) in human plasma by liquid chromatography electrospray tandem mass spectrometry (LC–ESI-MS/MS)
چکیده انگلیسی

A sensitive method for the determination of lapatinib (GW572016) in human plasma was developed using high-performance liquid chromatographic separation with tandem mass spectrometric detection. Plasma samples (100 μL) were prepared using solid phase extraction (SPE) columns, and 6.0 μL of the reconstituted eluate was injected onto a Phenomenex® CuroSil-PFP 3 μ analytical column (50 mm × 2.0 mm) with an isocratic mobile phase. Analytes were detected with a PE SCIEX API-365 LC–MS/MS system at unit (Q1) and low (Q3) resolution in positive multiple reaction monitoring mode (m/z 581 (precursor ion) to m/z 364 (product ion) for lapatinib). The mean recovery for lapatinib was 75% with a lower limit of quantification of 15 ng/mL (S/N = 11.3, CV ≤ 14%). This method was validated over a linear range of 100–10,000 ng/mL, and results from a 5-day validation study demonstrated good within-day and between-day precision and accuracy. This method has been used to measure plasma lapatinib concentrations in a Phase I study in children with cancer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 831, Issues 1–2, 2 February 2006, Pages 169–175
نویسندگان
, , , , ,