کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1217481 | 967102 | 2010 | 8 صفحه PDF | دانلود رایگان |
A novel analytical method has been developed to detect and quantify VX (O-ethyl S-(2(diisopropylamino) ethyl) (methylphosphonothioate)) in plasma using an LC–MS/MS technique. VX detection and quantification in plasma following percutaneous exposure represent a formidable challenge and it is an important part of the ongoing struggle against chemical warfare agents. Liquid–liquid extraction of VX from plasma was performed and it generated a recovery rate of approximately 65% followed by an LC–MS/MS analysis in a 100% organic phase. An Allure biphenyl column (Restek) was tested with detection limit at 0.5 pg/mL (5 μL injected). Initial application was focused on human skin grafted on nude mice as an experimental model with proper adjustments done for very small quantities of plasma.
Journal: Journal of Chromatography B - Volume 878, Issue 30, 15 November 2010, Pages 3059–3066