کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217562 967112 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of vitamin K1 in plasma by solid phase extraction and HPLC with fluorescence detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of vitamin K1 in plasma by solid phase extraction and HPLC with fluorescence detection
چکیده انگلیسی

We describe a procedure for quantification of vitamin K1 in human plasma by HPLC. Samples, enriched with a vitamin K derivative as internal standard, were deproteinized, purified on polymeric RP-SPE cartridges and injected into HPLC equipped with a post-column on-line zinc metal reactor and a fluorometric detector. Median level in blood donors (n = 87) was 1.967 nmol/L (0.93–4.01, 5th–95th percentiles), with a significant correlation between plasma levels and age (r = 0.276, p = 0.00958) and a lower (not significant) value in women than in men. This method, easy-to-handle and with a high throughput, can be used to identify covert states of vitamin K intake deficiency in patients thus at risk of alterations in blood clotting or bone mineralization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 877, Issue 3, 15 January 2009, Pages 351–354
نویسندگان
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