کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217656 1494175 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of levocetirizine in human plasma by liquid chromatography–electrospray tandem mass spectrometry: Application to a bioequivalence study
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of levocetirizine in human plasma by liquid chromatography–electrospray tandem mass spectrometry: Application to a bioequivalence study
چکیده انگلیسی

We describe a liquid chromatography–tandem mass spectrometric method (LC–MS/MS) for levocetirizine quantification (I) in human plasma. Sample preparation was made using a fexofenadine (II) addition as internal standard (IS), liquid–liquid extraction using cold dichloromethane, and dissolving the final extract in acetonitrile. I and II (IS) were injected in a C18 column and the mobile phase composed of acetonitrile:water:formic acid (80.00:19.90:0.10, v/v/v) and monitored using positive electrospray source with tandem mass spectrometry analyses. The selected reaction monitoring (SRM) was set using precursor ion and product ion combinations of m/z 389 > 201 for I and m/z 502 > 467 for II. The limit of quantification and the dynamic range achieved were 0.5 ng/mL and 0.5–500.0 ng/mL. Validation results on linearity, specificity, accuracy, precision and stability, as well as its application to the analysis of plasma samples taken up to 48 h after oral administration of 5 mg of levocetirizine dichloridrate in healthy volunteers demonstrate its applicability to bioavailability studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 862, Issues 1–2, 1 February 2008, Pages 132–139
نویسندگان
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