کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217657 1494175 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enantiospecific gas chromatographic–mass spectrometric analysis of urinary methylphenidate: Implications for phenotyping
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Enantiospecific gas chromatographic–mass spectrometric analysis of urinary methylphenidate: Implications for phenotyping
چکیده انگلیسی

A chiral derivatization gas chromatographic–mass spectrometric (GC–MS) method for urine methylphenidate (MPH) analysis was developed and validated to investigate preliminary findings regarding a novel MPH poor metabolizer (PM). Detection was by electron impact (EI) ionization-selected ion monitoring of the N-trifluoroacetylprolylpiperidinium fragments from MPH and the piperidine-deuterated MPH internal standard. The PM eliminated ∼70 times more l-MPH in urine (9% of the dose over 0–10 h), and ∼5 times more of the d-isomer (10% of the dose), than the mean values determined from 10 normal metabolizers of MPH. Only minor amounts of the metabolite p-hydroxy-MPH were found in the urine of both the PM and normal metabolizers, while the concentration of MPH lactam was not high enough to be detectable. The described method indirectly gauges the functional carboxylesterase-1 status of patients receiving MPH based on the evaluation of relative urine concentrations of d-MPH:l-MPH. Clinical implications concerning rational drug selection for an identified or suspected MPH PM are discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 862, Issues 1–2, 1 February 2008, Pages 140–149
نویسندگان
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