کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217729 1494178 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification of recombinantly expressed and cytotoxic human amyloid-beta peptide 1–42
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Purification of recombinantly expressed and cytotoxic human amyloid-beta peptide 1–42
چکیده انگلیسی

The amyloid cascade hypothesis assigns the amyloid-beta peptide (Aβ) a central role in the pathogenesis of Alzheimer's disease (AD). Although there are strong efforts to biophysically characterize formation of Aβ aggregates and fibrils, as well as their prevention, progress is still severly hampered by the availability of tens of milligrams of recombinant Aβ(1–42). Here, we describe a reliable and easy procedure to recombinantly express and purify Aβ(1–42), which is fully cytotoxic and able to form fibrils without any further refolding steps. The yield of the procedure is 5–8 mg of tag-less peptide per liter culture volume.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 856, Issues 1–2, 1 September 2007, Pages 229–233
نویسندگان
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