کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217747 1494178 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification of bovine hemoglobin via fast performance liquid chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Purification of bovine hemoglobin via fast performance liquid chromatography
چکیده انگلیسی

Bovine hemoglobin (bHb) was purified from bovine red blood cells (bRBCs) via anion exchange chromatography preceded by dialysis. This is a fast and effective way to obtain bHb from bRBCs using Q Sepharose XL, a strong anion exchange resin. This resin had double the binding capacity for bHb compared to three other anion exchange resins that were studied in this work. Methemoglobin levels remained below 2% with bHb concentrations between 0.7 and 1.7 mM. The high purity of bHb was confirmed via SDS-PAGE and size exclusion chromatography (SEC).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 856, Issues 1–2, 1 September 2007, Pages 353–357
نویسندگان
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