کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217749 1494178 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
On-line coupling of solid-phase extraction and capillary electrophoresis for the determination of cefoperazone and ceftiofur in plasma
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
On-line coupling of solid-phase extraction and capillary electrophoresis for the determination of cefoperazone and ceftiofur in plasma
چکیده انگلیسی

We present a method for determining two cephalosporins (cefoperazone and ceftiofur) in plasma by on-line solid-phase extraction (SPE)—capillary zone electrophoresis (CZE) with a T-split interface. Using this interface, a part of the SPE elution plug containing the cephalosporins is injected while the rest of the sample is flushed to waste. SPE was carried out using a C18 micro-precolumn and the cephalosporins presented good retention properties with breakthrough volumes above 1 ml. Using a desorption volume of 426 nl of acetonitrile, recoveries were 75 and 90%, for cefoperazone and ceftiofur, respectively. The resulting elution volume was about 1.8 μl. A deproteinization step was included prior to SPE for the analysis of plasma samples with recoveries of 90 and 57% for cefoperazone and ceftiofur, respectively. With UV detection at 254 nm, linear relationships between the injected concentration and peak area was measured between 10 and 500 ng ml−1 for standards, and 200 and 1500 ng ml−1 for plasma samples. Intra-day (n = 5) and inter-day (n = 5) peak area repeatability were lower than 12% RSD. The detection limits obtained for spiked plasma (100 ng ml−1 cefoperazone and ceftiofur) are sufficient for applying the method to pharmacokinetic studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 856, Issues 1–2, 1 September 2007, Pages 365–370
نویسندگان
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