کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217824 | 1494179 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 854, Issues 1–2, 1 July 2007, Pages 308–312
Journal: Journal of Chromatography B - Volume 854, Issues 1–2, 1 July 2007, Pages 308–312
نویسندگان
Cecilia Pedernera, José Luis Ruiz, Glòria Castells, Xavier Manteca, Carles Cristòfol,