کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1217886 | 967162 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and quantification of GC-rich oligodeoxynucleotides in tissue extracts by capillary gel electrophoresis
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کلمات کلیدی
ODNGC-richOligoribonucleotideCpG ODNORNoligodeoxynucleotideCpGNMFCGESPEDMSO - DMSOsiRNA - siRNAsmall interfering ribonucleic acid - اسید ریبونوکلئیک دخالت کندCapillary gel electrophoresis - الکتروفورز ژل مویرگیOligonucleotide - اولیگونوکلئوتیدDimethylsulfoxide - دیمتیل سولفواکسیدFormamide - فرمامید
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification and quantification of GC-rich oligodeoxynucleotides in tissue extracts by capillary gel electrophoresis Identification and quantification of GC-rich oligodeoxynucleotides in tissue extracts by capillary gel electrophoresis](/preview/png/1217886.png)
چکیده انگلیسی
Capillary gel electrophoresis (CGE) is a widely used method for quantification of oligonucleotide-based drugs, such as CpG oligodeoxynucleotides (CpG ODN), aptamers and small interfering ribonucleic acids (siRNAs) that allows accurate quantification of parent compound as well as metabolites. Stable secondary structure formation of these molecules frequently prevents analysis by conventional CGE methods and impedes pharmacokinetic assessment. Herein, we describe development of a CGE method for identification and quantification of complex mixtures of secondary structure forming GC-rich ODN in biological samples at dose levels of 0.5 mg/kg and above. Samples containing GC-rich CpG ODN and metabolite markers were treated by solid-phase-extraction (SPE) and subsequently analyzed by CGE using a 50 cm neutrally coated capillary at 60 °C together with a 7 M urea buffer system containing 30% dimethylsulfoxide (DMSO). Peak resolutions â¥1 were typically achieved, enabling pharmacokinetic assessment of secondary structure forming oligonucleotides in biological samples that hitherto were unsusceptible to quantitative analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 847, Issue 2, 1 March 2007, Pages 153-161
Journal: Journal of Chromatography B - Volume 847, Issue 2, 1 March 2007, Pages 153-161
نویسندگان
Bernhard O. Noll, Harald Debelak, Eugen Uhlmann,