کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1217905 967162 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Capillary electrophoretic assay for nitrate levels in the vitreous of proliferative diabetic retinopathy
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Capillary electrophoretic assay for nitrate levels in the vitreous of proliferative diabetic retinopathy
چکیده انگلیسی

The determination of nitric oxide (NO) in human vitreous samples is complicated by the relatively short half-life of the analyte and the viscous, high salt and protein biological matrix. In this work, we developed a fast (<5 min) and useful CE method to determine the stable metabolite, nitrate, from vitreous samples. This proposed method has been successfully applied to determine the nitrate levels from the vitreous humor of patients undergoing vitrectomy for a variety of conditions. A statistically significant increase (P = 0.000001) of the mean level of nitrate was observed in vitreous humor of patients with proliferative diabetic retinopathy (41.17 ± 4.09 μM, n = 27) versus controls (15.22 ± 0.86 μM, n = 35). The elevated levels of nitrate in the vitreous of patients known to have diabetic retinopathy suggests that NO is involved with the pathology of this disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 847, Issue 2, 1 March 2007, Pages 300–304
نویسندگان
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