کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1218114 1494496 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and validation of a specific method to quantify intact glucosinolates in honey by LC–MS/MS
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development and validation of a specific method to quantify intact glucosinolates in honey by LC–MS/MS
چکیده انگلیسی


• Intact glucosinolates (GLSs) were quantified in honey by LC–MS/MS for the first time.
• The proposed extraction method (dissolution and SPE) was efficient.
• Matrix did not have a significant influence on ESI–MS/MS signal.
• Low limits of quantification were obtained in all cases (<10 μg kg−1).
• Four intact GLSs were detected in some of the honey samples analyzed.

Glucosinolates (GLSs), which are secondary plant metabolites, have been scarcely investigated in honey. Recently, research has been conducted into the potential of GLSs as botanical biomarkers or as a parameter to establish honey freshness, but the employed methodologies were not specifically developed for intact GLSs quantification in honey. The aim of this study was to develop a new method to quantify twelve intact GLSs in honey, by using liquid chromatography with tandem mass spectrometry. An efficient extraction procedure was proposed; this involved dissolution of the honey with heated water, followed by solid-phase extraction with a weak anion exchange sorbent. Chromatography was performed on a C18 analytical column with a mobile phase of formic acid in water (0.5%, v/v) and formic acid in acetonitrile (0.5%, v/v). The method was fully validated in terms of selectivity, limits of detection (LOD) and quantification (LOQ), linearity, carry-over effect, reinjection reproducibility, precision and accuracy. The LOD and LOQ values were below 4 μg kg−1 and 10 μg kg−1, respectively. The proposed method was applied to analyze intact-GLSs in honey from different botanical origins. Four of the intact GLSs studied were identified at low concentration levels (<25 μg kg−1) in several of the samples analyzed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Composition and Analysis - Volume 46, March 2016, Pages 114–122
نویسندگان
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