کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1219590 | 967720 | 2015 | 13 صفحه PDF | دانلود رایگان |
• SF suppressed mitogen induced T and B-cell proliferation and cytokine secretion.
• SF increased basal ROS levels and depleted GSH/GSSG ratio in lymphocytes.
• Thiol antioxidants but not SOD/catalase abrogated SF mediated immunosuppression.
• SF induced phosphorylation of PI3K/AKT leading to inactivation of GSK3β.
• SF activated Nrf-2 and prevented direct interaction between NF-κB and DNA.
A potent Nrf-2 activator sulforaphane (SF), which is currently under clinical trials for cancer therapy, was employed to elucidate the role of Keap1/Nrf-2/ARE signalling pathway in T-cell responses. SF suppressed mitogen induced T-cell and B-cell proliferation. It also inhibited mitogen induced upregulation of T-cell (CD25 and CD69) and B-cell (CD80 and CD86) activation markers and suppressed secretion of cytokines (IL-2, IL-4, IL-6 and IFN-γ). SF induced oxidative stress and its anti-inflammatory effects were abrogated by thiol antioxidants. SF activated PI3K/AKT, leading to phosphorylation mediated suppression of GSK3β resulting in Nrf-2 activation. We also show for the first time that SF can prevent direct interaction between NF-κB and its consensus sequence by modulating thiol groups. It also suppressed homeostatic proliferation of T-cells and suppressed lipopolysaccharide induced pro-inflammatory mediators in macrophages. Our study shows that the potent anti-inflammatory effects of SF are mediated via modulation of PI3K/AKT/GSK3β/Nrf-2 and NF-κB pathway in T-cells.
Journal: Journal of Functional Foods - Volume 19, Part A, December 2015, Pages 426–438