Overall dietary polyphenol intake in a bowl of strawberries: The influence of Fragaria spp. in nutritional studies

• A total of 56 polyphenols were accurately quantified in a bowl of strawberries.
• Detailed de novo identification of some strawberry polyphenols.
• Precise estimation of strawberry composition for nutritional studies was achieved.

Strawberry (Fragaria × ananassa Duch.) is an important source of polyphenols in the human diet, and is considered a functional food. However, at the moment, it is not fully understood which compounds are involved. The aim was to present a comprehensive picture of the amount of polyphenols present in strawberry. A total of 56 individual compounds were identified and quantified with a triple-quadrupole mass spectrometer, their concentration ranging from 1 µg/100 g to 40 mg/100 g of fresh fruit. Furthermore, spectrophotometric assay for estimation of high-molecular mass proanthocyanidins was applied. Several compounds were identified/quantified for the first time.