کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1220194 1494611 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid analysis of Callicarpa L. using direct spray ionization mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rapid analysis of Callicarpa L. using direct spray ionization mass spectrometry
چکیده انگلیسی


• A novel mass spectrometry technique was applied in our works, named ambient ionization mass spectrometry.
• This paper reported on two means of ambient ionization mass spectrometry: leaf spray and paper spray.
• A leaf spray MS method to rapidly distinguish five species of Callicarpa L. without any sample preparation.
• A semi-quantitative method for phenylpropanoid glycosides in Callicarpa L. Folium extracts by mean of paper spray MS.
• The prominent merits of direct spray MS are simple, rapid(less than one minute) and low cost.

Direct spray such as leaf spray and paper spray ionization mass spectrometry (MS) is a powerful type of ambient MS for phytochemical analysis. In this paper, direct spray MS methods to rapidly distinguish and analyze five species of Callicarpa L. have been developed. To distinguish species, leaf spray MS was employed to directly analyze leaves. A small triangular leaf sample was wetted with 15 μL of spray solvent and a high DC voltage was then simply applied to the wet leaf sample, which was positioned in front of the inlet of a mass spectrometer to produce electrospray ionization. The MS signals of phenylpropanoid glycosides, i.e. forsythiaside B, poliumoside, verbascoside in leaves could be sensitively detected. The content characteristics of the phenylpropanoid glycosides in five species including Callicarpae kwangtungensis Folium, Callicarpae macrophyllae Folium, Callicarpa nudiflora Folium, Callicarpae formosanae Folium, Callicarpa longissima Folium could be used to distinguish them, then the mass spectra of the Callicarpa L. samples were analyzed using principal component analysis(PCA) or partial least squares-discriminant analysis(PLS-DA).For the rapid semi-quantitative analysis, of phenylpropanoid glycosides in leaves, paper spray MS was employed to determine phenylpropanoid glycosides in the extracts of Callicarpa L. leaves. Ginsenoside Rg1 was selected as an internal standard (I.S.). The calibration curves were constructed through ratios of target ion abundance to I.S. ion abundance vs. concentration of targets. The linearity range was 8–250 μg/mL (R2 = 0.9947) for forsythiaside B, 9–280 μg/mL (R2 = 0.9939) for verbascoside, and 9–260 μg/mL (R2 = 0.9917) for poliumoside, respectively. The limit of detection (LOD) was 1 μg/mL, 0.5 μg/mL and 1 μg/mL for forsythiaside B, verbascoside, and poliumoside, respectively.

Direct spray MS has been successfully applied for rapid analysis of phenylpropanoid glycosides, in less than one minute, in five species of Callicarpa L. Folium without any sample preparation. The MS profiles can be used to directly distinguish the five species. As a semi-quantitative method, paper spray MS is an available method for rapid evaluate the quality. The prominent merits of direct spray MS are simple, rapid, and low cost.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 124, 30 May 2016, Pages 93–103
نویسندگان
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