کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1220731 | 1494623 | 2015 | 10 صفحه PDF | دانلود رایگان |
• Both termini of basic fetoprotein (BFP) were sequenced by MALDI mass spectrometry.
• For identifying BFP, a terminal tag database, ProteinCarta, was employed.
• BFP was first identified as glucose-6-phosphate isomerase (G6PI_HUMAN).
Human basic fetoprotein (BFP), found in fetal serum and tissue extracts as well as in extracts of various cancer tissues, has long been known as a marker protein for cancers; however, the primary sequence has not yet been reported. This paper describes the identification of BFP using the N- and C-terminal amino acid sequence tags (Ac-AALTRDPQFQ and QQREARVQ, respectively) clarified by mass spectrometry-based methods, and a terminal tag database (ProteinCarta). In this study, BFP was identified as glucose-6-phosphate isomerase (G6PI_HUMAN).
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Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 112, 10 August 2015, Pages 116–125