کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1220977 | 1494636 | 2014 | 6 صفحه PDF | دانلود رایگان |
• Permethylated 6-monoamino-6-monodeoxy-βCD resolves dansylated amino acids.
• Maximal chiral resolutions could be achieved at pH 6 or pH 4.
• The separations improved with increasing concentration of the selector.
• Low CD concentration was enough for the separation of the most apolar Dns-AAs.
• Resolution power of PMMABCD was proved by the separation of Dns-AAs’ mixture.
The resolution power of permethylated 6-monoamino-6-monodeoxy-βCD (PMMABCD) – a single isomer, cationic CD derivative – developed previously for chiral analyses in capillary electrophoresis was further studied here. Dansylated amino acids (Dns-AA) were chosen as amphoteric chiral model compounds. Changes in the resolutions of Dns-AAs by varying pH and selector concentrations were investigated and correlated with their structures and chemical properties (isoelectric point and lipophilicity). Maximal resolutions could be achieved at pH 6 or pH 4. The separations improved with increasing concentration of the selector. Baseline or substantially better resolution for 8 pairs of these Dns-AAs could be achieved. Low CD concentration was enough for the separation of the most apolar Dns-AAs. Chiral discrimination ability of PMMABCD was demonstrated by the separation of an artificial mixture of 8 Dns-AA pairs.
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Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 99, October 2014, Pages 16–21