A fast and reliable method for GHB quantitation in whole blood by GC–MS/MS (TQD) for forensic purposes

• Fast, sensitive and reliable method for quantitation of GHB in whole blood.
• LOD and LLOQ of 0.1 mg/L.
• First GC–MS/MS method with different product ions for GHB and GHB-D6.
• Method comparable with more expensive UHPLC–MS/MS apparatus.

Gamma-hydroxybutyric acid (GHB) is an endogenous compound with a story of clinical use since the 1960s. However, due to its secondary effects, it has become a controlled substance, entering the illicit market.A fully validated, sensitive and reproducible method for the quantification of GHB by methanolic precipitation and GC–MS/MS (TQD) in whole blood is presented. Using 100 μL of whole blood, obtained results included a LOD and LLOQ of 0.1 mg/L and a recovery of 86% in a working range between 0.1 and 100 mg/L. This method is sensitive and specific to detect the presence of GHB in small amounts of whole blood (both ante-mortem or post-mortem), and is, to the authors’ knowledge, the first GC–MS–MS TQD method that uses different precursor ions and product ions for the identification of GHB and GHB-D6 (internal standard). Hence, this method may be especially useful for the study of endogenous values in this biological sample.

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