UPLC–Q-TOF-MS/MS fingerprinting of Traditional Chinese Formula SiJunZiTang

SiJunZiTang (SJZT), a classic Traditional Chinese Formula (TCMF) consisting of four herbs, Radix Ginseng, Atractylodes macrocephala, Poria cocos and Glycyrrhiza uralensis, has been demonstrated to show protective effects on intestine and stomach injure. The chromatographic quality control is needed. UPLC–Q-TOF-MS is a rapid and efficient technique for analysis of complex sample in combination with UPLC and tandem mass spectrometry (MS/MS). In this paper, UPLC–MS fingerprinting of SJZT was developed. As a result, 66 compounds including ginsenosides, flavonoids, triterpenoid and coumarins were detected, 58 of them were tentatively identified. The major constituents of SJZT were ginsenosides and flavonoids that coming from Radix Ginseng and Glycyrrhiza uralensis.

UPLC–Q-TOF-MS/MS chromatographic fingerprinting of Traditional Chinese Formula SiJunZiTang.Figure optionsDownload as PowerPoint slideHighlights
► UPLC/MS fingerprinting of SiJunZi Tang, a classic Traditional Chinese Formula, was developed.
► 66 compounds were detected and originality was confirmed.
► 58 compounds were identified by comparing the accurate mass and fragment information.
► The major constituents in SJZT extract came from Radix Ginseng and Glycyrrhiza uralensis.