کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1221484 1494646 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of lipid fraction constituents from grasshopper (Chorthippus spp.) abdominal secretion with potential activity in wound healing with the use of GC–MS/MS technique
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Identification of lipid fraction constituents from grasshopper (Chorthippus spp.) abdominal secretion with potential activity in wound healing with the use of GC–MS/MS technique
چکیده انگلیسی


• The acquisition of secretion from grasshopper abdomen.
• Extraction of compounds from the grasshopper abdominal secretion.
• GC separation of the prepared extracts and mass spectrometry data analysis.
• Chemometric data analysis and lipidome identification.

In recent years biologically active compounds isolated from insects call special interest of drug researchers. According to some Polish etnopharmacological observations, secretion from the grasshopper's abdomen (Orthoptera family) is believed to speed up the process of wound healing.In the present work we focused on determination of main components of the lipid fraction of material from grasshopper abdomen using GC–MS/MS. Samples were qualitatively analyzed using gas chromatography coupled with mass spectrometry. Both liquid–liquid extraction and solid-phase extraction pretreatment methods were used to concentrate and fractionate the compounds from the insect. In the derivatized fractions ca. 350 compounds were identified, including substances of known biological activity. The potential agents affecting wound healing have been indicated.A set of compounds characteristic for all the studied Chorthippus spp., have been identified. Data analysis revealed different lipidomic profiles of grasshoppers depending on the insects origin and collection area.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 89, 15 February 2014, Pages 56–66
نویسندگان
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