کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1221493 1494646 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of triamcinolone acetonide in silicone oil and aqueous humor of vitrectomized rabbits’ eyes: Application for a pharmacokinetic study with intravitreal triamcinolone acetonide injections (Kenalog® 40)
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of triamcinolone acetonide in silicone oil and aqueous humor of vitrectomized rabbits’ eyes: Application for a pharmacokinetic study with intravitreal triamcinolone acetonide injections (Kenalog® 40)
چکیده انگلیسی


• High-performance liquid chromatography method was developed and validated.
• Administration of TA injections in rabbits’ eyes.
• Method was successfully applied to quantify the drug in SO and aqueous humor.
• TA remained in SO and aqueous humor of rabbits’ eyes for 4 weeks.
• SO may play an important role in the elimination of lipophilic drugs.

A simple and accurate method including liquid–liquid extraction and protein precipitation procedures from silicone oil and aqueous humor samples followed by high-performance liquid chromatography (HPLC-UV) was developed and validated to determine the pharmacokinetic profile of triamcinolone acetonide in silicone oil and aqueous humor of rabbits’ eyes submitted to the pars plana vitrectomy surgery. The method was successfully applied to quantify the drug remaining in silicone oil and aqueous humor (LOQ range of 1 μg/mL). The triamcinolone acetonide remained in silicone oil and aqueous humor of vitrectomized rabbits’ eyes for four weeks after the intravitreal injections.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 89, 15 February 2014, Pages 24–27
نویسندگان
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