کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1222039 967853 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of rare flavonoid C-glycosides in Celtis australis L. by micellar electrokinetic chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Analysis of rare flavonoid C-glycosides in Celtis australis L. by micellar electrokinetic chromatography
چکیده انگلیسی

This manuscript reports on the first analytical procedure for the determination of flavonoids in Celtis australis. The capillary electrophoretic separation of 8 compounds, most of them flavone C-glycosides, was possible using a borax buffer with pH 9.0, which contained 25 mM SDS as detergent and 7.5% of n-butanol as organic modifier. Method validation revealed that the developed assay is repeatable (σrel ≤ 4.0%), precise (inter-day σrel ≤ 6.7%, intra-day σrel ≤ 3.9%), accurate (recovery rates from 96.8 to 102.3%), sensitive (LOD: 2.2–1.6 μg/ml) and linear (R2 ≥ 0.9996) within the tested concentration range. The quantitative analysis of several C. australis samples showed that isovitexin is the most abundant representative (0.06–0.09%), at a rather uniform content of total flavonoids of approx. 0.3% in all specimens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 51, Issue 5, 6 April 2010, Pages 1165–1168
نویسندگان
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