Enantioselectivity of polysaccharide-based chiral selectors in polar organic solvents chromatography: Implementation of chlorinated selectors in a separation strategy

The enantioselectivity of polysaccharide-based chiral stationary phases in polar organic solvents chromatography (POSC) is investigated using both chlorinated and non-chlorinated selectors. A test set of 58 drug compounds was analyzed at the screening conditions of a previously developed separation strategy. Eight stationary phases were screened with eight mobile phases, resulting in 64 chromatographic systems. After selection of the two most successful mobile phases, a preferred testing sequence for the polysaccharide selectors was determined, based on their success rate and complementarity. These stationary phases, screened in the sequence Lux Cellulose-2 > Chiralcel OD-RH > Lux Cellulose 4 > Sepapak-5, each with two mobile phases, showed 48/58 separations (83% of the test set). An attempt was also made to update the existing separation strategy. The existing screening conditions were found to be easily applicable on stationary phases with chlorinated selectors, but the optimization steps appeared to be less successful.