کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1222114 | 1494673 | 2012 | 5 صفحه PDF | دانلود رایگان |

A new high-performance liquid chromatography/tandem mass spectrometry (LC–MS/MS) was developed for quantitative analysis of atractylenolide I in rat plasma using buspirone as internal standard (I.S.). Rat plasma samples were deproteined with methanol and acetonitrile (1:1, v/v). Atractylenolide I and I.S. were separated on a Phenomenex Gemini C18 column (50 mm × 2.0 mm, 5 μm) with gradient mobile phase at the flow rate of 0.4 ml/min. The detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The linear calibration curve of atractylenolide I in rat plasma ranged 2.0–5000 ng/ml (R > 0.9979). The limit of detection (LOD) and the limit of quantification (LOQ) were 0.6 ng/ml and 2.0 ng/ml, respectively. Both accuracy and precision of the assay were satisfactory. The recoveries of atractylenolide I and I.S. were 91.4% and 87.8%, respectively. This fully validated method was applied to a pharmacokinetic study of atractylenolide I in rats administered with 20 g/kg Atractylodis extract. The main pharmacokinetic parameters Tmax (the time to peak), Cmax (the concentration to peak), T0.5 (the biological half time), and Ke (the elimination rate constant) were 0.81 ± 0.11 h, 7.99 ± 1.2 ng/ml, 1.94 ± 0.27 h, 0.365 ± 0.06/h, respectively.
► We develop an HPLC/MS method for quantification of atractylenolide I in rat plasma.
► Pharmacokinetics was performed in rats ingested with 20 g/kg Atractylodis extract.
► Atractylenolide I was found to be absorbed quickly within 1 h.
► The biological half-life and the elimination rate constant of atractylenolide I were 1.94 ± 0.27 h and 0.365 ± 0.06 h, respectively.
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 58, 25 January 2012, Pages 172–176